Dear Mr Ogundeyi,
Thank you for your recent enquiry.
You do not mention the upper and lower pressure limits to which you have set the pump. As Bill has mentioned, you may have set the upper pressure limit at too low a value.
Please be aware that pump over pressure, may be also due to:-
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The analytical or guard column being too ‘dirty’ or clogged with debris.
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A blockage in the LC system tubing/mobile phase. Is your mobile phase filer clean, should your mobile phase be filtered.
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Too high a flow rate for the analytical and guard columns.
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Inadequate degassing of the mobile phase.
Please check out the above points.
If you have any further queries, please contact me directly, and provide your full contact details.
Kind regards,
Ade Kujore
Marketing
Cecil Instruments Limited
Milton Technical Centre, Cambridge CB24 6AZ United Kingdom
email:- ade.kujore@cecilinstruments.com
telephone:- +44 (0) 1223 420821
fax.:- +44 (0) 1223 420475
web site:- www.cecilinstruments.com
Registered Office: Unex House, Bourges Boulevard, Peterborough PE1 1NG,
United Kingdom Registered Number 909536
Cecil AutoQuest LC autosamplers, for elegantly effective sampling.
http://www.cecilinstruments.com/adept-autosamplers.html