Discussion Category:  LC-MS Systems

No peak in negative mode in LC-MS

Hi all
I can't tune my agilent 6410 LC-MS in negative mode. Due to missing low mass fragments, Autotune is not completed and I must use manual tune instead. I have no problem with positive mode, but there is no any characteristic ion in low mass range in negative mode. I tried with target tune (with several analytes) but I can't find any fragment. All parts of the instrument were cleaned several times but the problem is persist.

A screenshot of manual tune page (in both positive and negative modes) could be viewed on the following links:

positive mode:

https://drive.google.com/file/d/0B9GH9d ... sp=sharing

Negative mode:

https://drive.google.com/file/d/0B9GH9d ... sp=sharing

Any help would be appreciated.

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Asked by

Alc.John
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