Discussion Category:  HPLC Systems

Trying to translate method from Waters 2690 to Dionex u3000

I am new to LC work and am not sure where to begin. I need to translate a method from an old Waters 2690 with DAD detector to a newer u3000 LC with DAD. I used the same method for the gradient analysis on both instruments, but am getting about double the absorbance units on the newer instrument for the same sample. If the cell path length is the same, shouldn't the AUs be the same? What else can I check to see what could be causing the difference?

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karrun
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