Discussion Category:  FPLC – Fast Protein Liquid Chromatography

No pressure

Hi we recently used our Acta Prime after not using it for 2 months...The washing was ok and when it's time for the smaple, suddenly no pressure was observed and the sample were not taken up..Try to start again, still no pressure observed.What should we do.Thank you

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nab
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BioSciSolution

Hi nab, 

Are you using the pump to load your sample? of the injection valve? 

BioSciSolutions

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