Discussion Category:  PCR / Thermal Cyclers

ABI 7900 error 10077 Thermal cycler not responding ot set cover temp.

Hi all,

 

I am having errors with our 7900HT when trying to run PCRs. Usually the machine runs taqman protocol, but when we try to change to SYBR green assay it gives this error:

"Unable to load the plate due to an instrument error:10077 : Error. Thermal cycler not responding to set cover temperature."

Any help with resolving this would be greatly appreciated

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