Discussion Category:  GPC Gel Permeation Chromatography

Autozero doesnot zero the RI

I work on DMF, 80 degrees, try purging many times the RI and autozero is at -0.132V constant, so I cannot start running samples....

Any advice?

Thanks

Moris

 

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chmoris
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chromatology

Have you purged the reference side of the flow cell with your mobile phase first?

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chmoris

Yes indeed, I have no columns at the moment and washing with 1 ml/min at a 1.3 Mbar. Washing reference size, pressure goes down to 0.7 Mbar

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