Discussion Category: HPLC Systems
Pump gradient does not run and pump stuck in isocratic elution
In the pump method I specify a gradient elution, and the method is shown in the acquisition panel, but the pump is not doing the gradient, it just remains on the initial solvent condition. I can manually carry out a gradient using the control flow button and simply enter values in real time, and the pump responds, but it doesn't carry out the elution condtions specified in the pump method. I suspect it is a problem with how my method is set up, but I am stuck and would rather not pay $300 an hour for a technical service rep to talk on the phone.
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