Discussion Category: Microplate Readers
filter in Wallac 1420
Hi, I am a researcher at University of Guelph. We have a Wallac 1420 Victor 2 in our lab. I want to ask you a question about using this instrument.
Thank you very much for your reply. I am going to use it for RNA detection. The RNA copy in my sample will be amplified by a RNA polymerase, and a fluorescence dye-SYBR Green II will be added, so with the amplification of RNA, the fluorescence will become stronger and stronger. I would like to use this instrument to test the fluorescence.
Now, I meet two questions. The first one is the filter. The excitation wavelength of SYBR Green II is optimal at 497nm, and emission wavelength is optimal at 521nm. But we only have 485/535nm in our machine. So, do you have that filter I need? If you have the filter, I would like to buy it. The second question is that what is the difference between time-resoved fluorescence, fluorescence polarization, and lance. For me, could you please give me suggestion? Thanks very much for your reply.
Best wisehs,
Ruiqin