Discussion Category: LC-TOF MS
Analysis of protein isolated using TriReagent protocol
Hi, I wonder will there be any huge effect on the mass spectra if my protein to be analyse was extracted using TriReagent protocol. My concern is that the protocol use 2.5% glycerol for washing while we know glycerol is not compatible with the system. I'm quite confused here, is the buffer compatibility refer to the final elution or every buffer being used throughout the extraction?
Thanks in advance!