Discussion Category: GPC Gel Permeation Chromatography
Problem with the light scattering detector
I am facing a problem with the light scattering detector on my GPC. I removed the columns and connetced the pump to the light scattering detector and flushed first Decalin for 48 hours at 160 degrees, then flushed the detector with acetone at 40 degrees and then moved back to 160 degrees and fulshed TCB. The detecor response still showing 400mV (LS15) and 220mv (LS90). Any thought of how I can solve this high response.
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